Product Description

CENPH Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES9252-50, ES9252-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human protein. at AA range: 1-80

Isotype: IgG

Target: CENPH

Specificity: CENPH Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Cervix carcinoma, Placenta, Testis,

Observed Band (KD): 27kD

Function: Function: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres
Similarity: Belongs to the centromere protein H family
Subcellular Location: Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase
Subunit: Self-associates. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with KIF2

Subcellular Location: Nucleus Chromosome, centromere, kinetochore Associates with active centromere-kinetochore complexes throughout the cell cycle Colocalizes with inner kinetochore plate proteins CENPA and CENPC during both interphase and metaphase

Storage and Stability: -20°C/1 year

Gene Name: CENPH ICEN35

Protein Name: Centromere protein H (CENP-H) (Interphase centromere complex protein 35)

Mouse SwissProt Number: Q9QYM8

Research Use Only