CENTRI-SPIN 10
Centri-Spin 10 spin columns are used for:
- electroporation nick translation binding applications
- FISH transfection probe labeling gene products
- signal transduction differential display antibody labeling transformation studies
- protein labeling receptor analysis
- solid phase array desalting.
Centri-Spin 10 spin columns are used for the fast and efficient purification of larger molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.)
The Centri-Spin 10 gel will provide excellent recovery of DNA fragments with sizes greater than 10 base pairs or 10-mer, while removing > 98 % of salts, NTP's and other unwanted low-molecular-weight impurities. Proteins, peptides, and protein conjugates > 5 kD can also be efficiently separated/desalted from unwanted low-molecular-weight impurities.
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Spin 10 unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
The column gel is hydrated with either reagent-grade water or a suitable buffer, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the interstitial fluid. After your sample is applied, the column is spun again to remove the low-molecular-weight components, leaving you not only with a purified sample, but one exchanged into the buffer of your choice.
CENTRI-SPIN 10 COLUMNS ALSO CAN BE USED FOR THE FOLLOWING APPLICATIONS:
- Purification of primers or probes > 10 bases
- Purification of biotinylation reactions
- Purification of protein conjugates in:
- iodination
- cross-linking
- fluorescence labeling
- haptenation
- Desalting / purification / buffer exchange of peptides or proteins > 5 kD prior to mass spectroscopy.
These columns are far superior – in their ease of use, speed, and non-toxicity – to such common techniques as phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration.
ADVANTAGES OF CENTRI-SPIN 10:
- Processed faster than ultrafiltration columns
- Rapid and efficient separations
- Buffer is not preselected
- Convenient 20–50 µL sample size
- Dry gel format
- Room Temperature Storage
- Microcentrifuge format
BENEFITS OF CENTRI-SPIN 10:
- Reliable and consistent
- Time-saving and convenient
- Enhanced stability
- Extended shelf-life
- No special equipment required
- Extremely versatile