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Centri-Spin 10 - CS-100/CS-101

Centri-Spin 10 - CS-100/CS-101

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$62.00 USD
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CENTRI-SPIN 10

Catalogue Numbers: CS-100 (Box of 20 Columns), CS-101 (Box of 50 Columns)
Sizes: Box of 20 Columns (CS-100), Box of 50 Columns (CS-101)
Protocol Available Here.
 

Centri-Spin 10 spin columns are used for:

  • electroporation nick translation binding applications
  • FISH transfection probe labeling gene products
  • signal transduction differential display antibody labeling transformation studies
  • protein labeling receptor analysis
  • solid phase array desalting.

Centri-Spin 10 spin columns are used for the fast and efficient purification of larger molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.)

The Centri-Spin 10 gel will provide excellent recovery of DNA fragments with sizes greater than 10 base pairs or 10-mer, while removing > 98 % of salts, NTP's and other unwanted low-molecular-weight impurities. Proteins, peptides, and protein conjugates > 5 kD can also be efficiently separated/desalted from unwanted low-molecular-weight impurities.

The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Spin 10 unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.

The column gel is hydrated with either reagent-grade water or a suitable buffer, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the interstitial fluid. After your sample is applied, the column is spun again to remove the low-molecular-weight components, leaving you not only with a purified sample, but one exchanged into the buffer of your choice.

CENTRI-SPIN 10 COLUMNS ALSO CAN BE USED FOR THE FOLLOWING APPLICATIONS:

  • Purification of primers or probes > 10 bases
  • Purification of biotinylation reactions
  • Purification of protein conjugates in:
  1. iodination
  2. cross-linking
  3. fluorescence labeling
  4. haptenation
  • Desalting / purification / buffer exchange of peptides or proteins > 5 kD prior to mass spectroscopy.

These columns are far superior – in their ease of use, speed, and non-toxicity – to such common techniques as phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration.

ADVANTAGES OF CENTRI-SPIN 10:

  • Processed faster than ultrafiltration columns
  • Rapid and efficient separations
  • Buffer is not preselected
  • Convenient 20–50 µL sample size
  • Dry gel format
  • Room Temperature Storage
  • Microcentrifuge format

BENEFITS OF CENTRI-SPIN 10:

  • Reliable and consistent
  • Time-saving and convenient
  • Enhanced stability
  • Extended shelf-life
  • No special equipment required
  • Extremely versatile