Centri-Spin 40 spin columns are used for:

• electroporation nick translation binding applications
• FISH transfection probe labeling gene products
• signal transduction differential display
• antibody labeling transformation studies
• protein labeling receptor analysis
• solid phase array desalting

Centri-Spin 40 spin columns are used for the fast and efficient purification of larger molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.)

Centri-Spin 40 gel will retain up to 80 % of short oligonucleotides (< 25-mer) while providing excellent recovery of up to 70 % of large DNA fragments with sizes greater than 135 base pairs. Proteins, peptides, and protein conjugates > 100 kD can also be efficiently separated from unwanted lower molecular-weight impurities.

The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. 1 Each Centri-Spin 40 unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.

The column gel is hydrated with either reagent-grade water or a suitable buffer, and spun in a microcentrifuge or swinging-bucket Centrifuge to remove the interstitial fluid. After your sample is applied, the column is spun again to remove the low-molecular-weight components, leaving you not only with a purified sample, but one exchanged into the buffer of your choice.

CENTRI-SPIN 40 COLUMNS ALSO CAN BE USED FOR THE FOLLOWING APPLICATIONS:

• Centri-Spin 40 columns have been designed specifically for the following applications:
• Removal of primers or primer-dimers from PCR extension products > 150 base pairs
• Desalting and purification of proteins > 100 kD
• Removal of the following impurities from solution:

1. transferrin
2. BSA
3. hemoglobin
4. albumin

ADVANTAGES OF CENTRI-SPIN 40:

• Processed faster than ultrafiltration columns
• Rapid and efficient separations
• Buffer is not preselected
• Convenient 20–50 µL sample size
• Dry gel format
• Room Temperature Storage
• Microcentrifuge format

BENEFITS OF CENTRI-SPIN 40:

• Reliable and consistent
• Time-saving and convenient
• Enhanced stability
• Extended shelf-life
• No special equipment required
• Extremely versatile