Product Description

Chicken APEX1 (Apurinic/Apyrimidinic Endonuclease 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8968-48, ELK8968-96, ELK8968-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken APEX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken APEX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken APEX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken APEX1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: APE, APE1, APEN, APEX, APX, HAP1, REF1, Multifunctional DNA Repair Enzyme, APEX Nuclease 1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase

Reactivity: Chicken

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase

Assay Length: 3.5h

Sensitivity: 0.149 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Research Use Only