Chicken GC (Glucagon) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK5744-48, ELK5744-96, ELK5744-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone
Assay Type: Competitive Inhibition
Sensitivity: 7.53 pg/mL
Standard: 1500 pg/mL
Detection Range: 23.44-1500 pg/mL
Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken GC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P68259
Research Use Only