Chicken GnRH (Gonadotropin Releasing Hormone) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8495-48, ELK8495-96, ELK8495-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
Assay Type: Competitive Inhibition
Sensitivity: 0.1 ng/mL
Standard: 16 ng/mL
Detection Range: 0.25-16 ng/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology; Reproductive science; Neuroscience; Genetic science; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken GnRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P37042
Research Use Only