Product Description

Chicken NPT (Neopterin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9161-48, ELK9161-96, ELK9161-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken NPT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken NPT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken NPT in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Neopterin

Reactivity: Chicken

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Cytokine, Infection Immunity

Assay Length: 2h

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Research Use Only