Chicken T4 (Thyroxine) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8957-48, ELK8957-96, ELK8957-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Assay Type: Competitive Inhibition
Sensitivity: 1.49 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken T4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only