Chicken TBARS (Thiobarbituric Acid Reactive Substance) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK11258-48, ELK11258-96, ELK11258-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: TBARS; Thiobarbituric Acid Reactive Substance
Assay Type: Competitive Inhibition
Sensitivity: 3.56 nmol/mL
Standard: 400 nmol/mL
Detection Range: 6.25-400 nmol/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 2h
Research Areas: Metabolic pathway; Hepatology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken TBARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only