Chicken TSH (Thyroid Stimulating Hormone) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9301-48, ELK9301-96, ELK9301-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Thyrotropin
Assay Type: Sandwich
Sensitivity: 19.3 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Endocrinology; Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken TSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken TSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken TSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken TSH in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only