Product Description

CHP2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1979-50, ES1979-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene product is a small calcium-binding protein that regulates cell pH by controlling plasma membrane-type Na+/H+ exchange activity. This protein shares sequence similarity with calcineurin B and can bind to and stimulate the protein phosphatase activity of calcineurin A (CnA) and functions in the calcineurin/NFAT (nuclear factor of activated T cells) signaling pathway. Another member of the CHP subfamily, Calcineurin B homologous protein 1, is located on Chromosome 15 and is an inhibitor of calcineurin activity and has a genetic phenotype associated with Parkinson's Disease (OMIM:606988). This gene was initially identified as a tumor-associated antigen and was previously referred to as Hepatocellular carcinoma-associated antigen 520. [provided by RefSeq, Jul 2013],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CHP2, HCA520, Calcineurin B homologous protein 2, Hepatocellular carcinoma-associated antigen 520

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CHP2. AA range:101-150

Isotype: IgG

Target: CHP2

Specificity: CHP2 Polyclonal Antibody detects endogenous levels of CHP2 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in malignantly transformed cells but not detected in normal tissues,

Observed Band (KD): 22kD

Function: Function: Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death
Similarity: Contains 4 EF-hand domains
Subunit: Binds to SLC9A1/NHE1
Tissue Specificity: Expressed in malignantly transformed cells but not detected in normal tissues

Subcellular Location: Nucleus Cytoplasm Cell membrane Predominantly localized in a juxtanuclear region Colocalizes with SLC9A3 in the juxtanuclear region and at the plasma membrane (By similarity) Exported from the nucleus to the cytoplasm through a nuclear export signal (NES) pathway May shuttle between nucleus and cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: CHP2

Protein Name: Calcineurin B homologous protein 2

Mouse SwissProt Number: Q9D869

Rat Gene ID: 308965

Rat SwissProt Number: Q810D1

Research Use Only