Product Description

CKAP2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES17423-50, ES17423-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a cytoskeleton-associated protein that stabalizes microtubules and plays a role in the regulation of cell division. The encoded protein is itself regulated through phosphorylation at multiple serine and threonine residues. There is a pseudogene of this gene on chromosome 14. Alternative splicing results in multiple transcript variations. [provided by RefSeq, Nov 2013],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human CKAP2 AA range: 298-348

Isotype: IgG

Target: CKAP2

Specificity: This antibody detects endogenous levels of CKAP2 at Human/Mouse

Dilution: WB 1：500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney,

Molecular Weight (Da): 75kD

Function: Developmental Stage: Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C)
Function: Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner
Induction: Up-regulated in primary human gastric cancers
Sequence Caution: Contaminating sequence. Potential poly-A sequence
Similarity: Belongs to the CKAP2 family
Subcellular Location: Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle micr

Subcellular Location: Cytoplasm, cytoskeleton Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2 At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules During cytokinesis, absent from midbody microtubules

Storage and Stability: -20°C/1 year

Gene Name: CKAP2 LB1 TMAP

Protein Name: CKAP2

Mouse Gene ID: 80986

Mouse SwissProt Number: Q3V1H1

Research Use Only