Product Description

CKAP5 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES17422-50, ES17422-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation, it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human CKAP5 AA range: 1446-1496

Isotype: IgG

Target: CKAP5

Specificity: This antibody detects endogenous levels of CKAP5 at Human/Mouse

Dilution: WB 1：500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Overexpressed in hepatomas and colonic tumors, Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas, Expression is elevated in the brain, highly expressed in the Purkinje cell bodies of the cerebellum,

Molecular Weight (Da): 224kD

Function: Function: Plays a major role in organizing spindle poles
Sequence Caution: Contaminating sequence. Potential poly-A sequence
Similarity: Belongs to the TOG/XMAP215 family
Similarity: Contains 10 HEAT repeats
Subunit: Interacts with TACC1
Tissue Specificity: Over-expressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas

Subcellular Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle pole Cytoplasm, cytoskeleton, spindle Chromosome, centromere, kinetochore Detected on centrosomes and kinetochores during interphase and mitosis independently from TACC3 and clathrin Located to spindle poles and microtubules during mitosis In complex with TACC3 localized to microtubule plus-ends in mitosis and interphase In complex with TACC3 and clathrin localized to inter-microtubule bridges in mitotic spindles Accumulation sites at microtubule plus ends protruded approximately 100 nm from MAPRE1/EB1 sites in interphase cells

Storage and Stability: -20°C/1 year

Gene Name: CKAP5 KIAA0097

Protein Name: CKAP5

Mouse Gene ID: 75786

Mouse SwissProt Number: A2AGT5

Research Use Only