Product Description

Cleaved-Caspase-4/5 p20 (D270/D311) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7702-50, ES7702-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

Reactivity: Human

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CASP4, ICH2, Caspase-4, CASP-4, ICE(rel)-II, Protease ICH-2, Protease TX, CASP5, ICH3, Caspase-5, CASP-5, ICE(rel)-III, Protease ICH-3, Protease TY

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 4/5. AA range:221-270

Isotype: IgG

Target: Caspase-4/5

Specificity: Cleaved-Caspase-4/5 p20 (D270/D311) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-4/5 p20 protein resulting from cleavage adjacent to D270/D311.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level), Not detected in brain,

Observed Band (KD): 47,22kD

Fields: Neutrophil extracellular trap formation, NOD-like receptor signaling pathway, Pathogenic Escherichia coli infection, Shigellosis, Salmonella infection

Function: Catalytic Activity: Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-
Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1
PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8
Similarity: Belongs to the peptidase C14A family
Similarity: Contains 1 CARD domain
Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit
Tissue Specificity: Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain

Subcellular Location: Cytoplasm, cytosol Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side Mitochondrion Inflammasome Secreted Predominantly localizes to the endoplasmic reticulum (ER) Association with the ER membrane requires TMEM214 (PubMed:15123740) Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630)

Storage and Stability: -20°C/1 year

Gene Name: CASP4

Protein Name: Caspase4

Research Use Only