Product Description

CLIA Kit for Amphiregulin (AREG)

Sizes: 24T, 48T, 96T

Catalogue Numbers: SCA006Ra-24, SCA006Ra-48, SCA006Ra-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Featured Series Function: Detects protein (regular version)

Method: Chemiluminescence

Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Amphiregulin (AREG) level in the sample or standard.;

Reactivity: Rat

Alternate Names: AR, CRDGF, SDGF, Colorectum Cell-Derived Growth Factor, Schwannoma-Derived Growth Factor

Target: Amphiregulin,

Assay Length: 2h, 40min

Sample Size: 100uL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Detection Method: Double-antibody Sandwich

Detection Range: 1.37-1000pg/mL

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amphiregulin (AREG) were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amphiregulin (AREG) were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Assay Procedure: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C; 8. Read RLU value immediately.

Specificity: Reactive with Rat AREG / Amphiregulin

Sensitivity: 0.58pg/mL

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Shelf Life: 12 months

Gene ID: 29183

UniProt: P24338

Research Use Only