Product Description

CLIA Kit for Apelin 12 (AP12)

Sizes: 24T, 48T, 96T

Catalogue Numbers: CCA856Hu-24, CCA856Hu-48, CCA856Hu-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Featured Series Function: Detects small molecule

Method: Chemiluminescence

Test Principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Apelin 12 (AP12). A competitive inhibition reaction is launched between biotin labeled Apelin 12 (AP12) and unlabeled Apelin 12 (AP12) (Standards or samples) with the pre-coated antibody specific to Apelin 12 (AP12). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin 12 (AP12) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Apelin 12 (AP12) level in the sample or standard.

Reactivity: Human

Alternate Names: APLN12

Target: Apelin 12,

Assay Length: 2h

Sample Size: 50uL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Detection Method: Competitive Inhibition

Detection Range: 62.5-16,000pg/mL

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin 12 (AP12) were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin 12 (AP12) were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Assay Procedure: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C; 7. Read RLU value immediately.

Specificity: Reactive with Human AP12 / Apelin 12

Sensitivity: 22.8pg/mL

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Shelf Life: 12 months

Gene ID: 8862

UniProt: Q9ULZ1

Research Use Only