Product Description

CLIA Kit for Calcitonin Gene Related Peptide (CGRP)

Sizes: 24T, 48T, 96T

Catalogue Numbers: CCA876Hu-24, CCA876Hu-48, CCA876Hu-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Featured Series Function: Detects small molecule

Method: Chemiluminescence

Test Principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Calcitonin Gene Related Peptide (CGRP). A competitive inhibition reaction is launched between biotin labeled Calcitonin Gene Related Peptide (CGRP) and unlabeled Calcitonin Gene Related Peptide (CGRP) (Standards or samples) with the pre-coated antibody specific to Calcitonin Gene Related Peptide (CGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Calcitonin Gene Related Peptide (CGRP) level in the sample or standard.

Reactivity: Human

Alternate Names: CALCB, CGRP2, CALC2, CGRP-II, Calcitonin Gene Related Peptide 2, Calcitonin 2, Calcitonin Related Polypeptide Beta

Target: Calcitonin Gene Related Peptide,

Assay Length: 2h

Sample Size: 50uL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Detection Method: Competitive Inhibition

Detection Range: 7.8-2,000pg/mL

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Assay Procedure: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C; 7. Read RLU value immediately.

Specificity: Reactive with Human CGRP / Calcitonin Gene Related Peptide

Sensitivity: 2.9pg/mL

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Shelf Life: 12 months

Gene ID: 797

UniProt: P10092

Research Use Only