Product Description

CLIC5 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES17374-50, ES17374-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the chloride intracellular channel (CLIC) family of chloride ion channels. The encoded protein associates with actin-based cytoskeletal structures and may play a role in multiple processes including hair cell stereocilia formation, myoblast proliferation and glomerular podocyte and endothelial cell maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human CLIC5 AA range: 156-206

Isotype: IgG

Target: CLIC5

Specificity: This antibody detects endogenous levels of CLIC5 at Human/Mouse/Rat

Dilution: WB 1：500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed in both fetal and adult human tissues (PubMed:24781754), Isoform 1 is expressed in renal glomeruli endothelial cells and podocytes (at protein level),

Molecular Weight (Da): 45kD

Function: Domain: Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion
Function: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti
Similarity: Belongs to the chloride channel CLIC family
Similarity: Contains 1 GST C-terminal domain
Subcellular Location: Associates with the cortical actin cytoskeleton. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain
subcellular loc

Subcellular Location: [Isoform 1]: Cytoplasm, cytoskeleton Cytoplasm, cell cortex Membrane, Single-pass membrane protein Apical cell membrane, Single-pass membrane protein Cytoplasm Associates with the cortical actin cytoskeleton (PubMed:10793131, PubMed:15184393) Localizes to the apical region of cochlear hair cells, at the base of the actin-rich hair bundle (By similarity) Colocalizes with podocalyxin at the apical cell membrane in renal glomeruli (PubMed:20335315) May localize to the centrosome in lens epithelial cells (By similarity) Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity), [Isoform 2]: Golgi apparatus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Colocalizes with AKAP9 at the G

Storage and Stability: -20°C/1 year

Gene Name: CLIC5

Protein Name: CLIC5

Mouse Gene ID: 224796

Mouse SwissProt Number: Q8BXK9

Rat Gene ID: 94272

Rat SwissProt Number: Q9EPT8

Research Use Only