Product Description

CML (Carboxymethyl Lysine) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7896-48, ELK7896-96, ELK7896-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CML. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CML. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CML in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: N(6)-Carboxymethyllysine

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Metabolic Pathway, Endocrinology, Cardiovascular Biology, Genetic Science, Nutrition Metabolism

Assay Length: 2h

Sensitivity: 24.7 ng/mL

Standard: 5000 ng/mL

Detection Range: 78.13-5000 ng/mL

Research Use Only