Product Description

COP ζ1 (I48) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3287-50, BS3287-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P61923

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Membrane and vesicular trafficking in the early secretory pathway are mediated by non-Clathrin COP (coat protein) I-coated vesicles. COPI-coated vesicles mediate retrograde transport from the Golgi back to the ER and intra-Golgi transport. The cytosolic precursor of the COPI coat, the heptameric coatomer complex, is composed of two subcomplexes. The first consists of the COPB, COPG, COPD and COPZ1 subunits (also known as β-COP, γ-COP, δ-COP and ζ-1 COP, respectively), which are distantly homologous to AP Clathrin adaptor subunits. The second consists of the COPA, COPP and COPE subunits (also known as α-COP, COPP and ε-COP, respectively).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: COP ζ1 (I48) polyclonal antibody detects endogenous levels of COP ζ1 protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of COP ζ1 (I48) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: HEK293T cell lysate
Lane 3: sp2/0 cell lysate
Lane 4: H9C2 cell lysate
Lane 5: PC12 cell lysate, Immunohistochemistry (IHC) analyzes of COP ζ1 (I48) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:5000. Nucleus speckle and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of COP ζ1 (I48) pAb in paraffin-embedded human stomach carcinoma tissue at 1:5000. Nucleus speckle and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Coatomer subunit zeta-1; Zeta-1-coat protein; Zeta-1 COP; COPZ1; COPZ; CGI-120; HSPC181; COP ζ-1

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human COP ζ1.

Conjugate: Unconjugated

Modification: Unmodified