Product Description

CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Sizes: 50Assays, 100Assays

Catalogue Numbers: E-BC-K022-S-50, E-BC-K022-S-100

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Superoxide anion (O2-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

Abbreviation: CuZn-SOD

Target Synonym: CuZn-SOD;T-SOD

Research Areas: Oxidative Stress

Result Type: Enzyme activity

Detection Instrument: Spectrophotometer

Detection Type: Colorimetric method

Detection Range: 2.03-155 U/mL

Tested Sample Types: Serum;Plasma;Urine;Cell;Cell culture supernatant;Tissue

Assay Length: 1 h 40 min

Sensitivity: 2.03 U/mL

Inter cv: 7.2%

Intra cv: 4.0%

Research Use Only