Product Description

Cyclin D3 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7915-50, ES7915-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008],

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CCND3, G1/S-specific cyclin-D3

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin D3. AA range:243-292

Isotype: IgG

Target: Cyclin D3

Specificity: Cyclin D3 Polyclonal Antibody detects endogenous levels of Cyclin D3 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Lung, Placenta, Synovial membrane tissue, Umbilical cord blood,

Observed Band (KD): 32kD

Fields: Cell cycle, p53 signaling pathway, PI3K-Akt signaling pathway, Cellular senescence, Wnt signaling pathway, Hippo signaling pathway, Focal adhesion, JAK-STAT signaling pathway, Measles, Influenza A, Human papillomavirus infection, Human T-cell leukemia virus 1 infection, Epstein-Barr virus infection, Pathways in cancer, Viral carcinogenesis, Chemical carcinogenesis - receptor activation

Function: Function: Essential for the control of the cell cycle at the G1/S (start) transition. Potentiates the transcriptional activity of ATF5
Similarity: Belongs to the cyclin family
Similarity: Belongs to the cyclin family. Cyclin D subfamily
Subunit: Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K

Subcellular Location: Nucleus Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: CCND3

Protein Name: G1/S-specific cyclin-D3

Mouse Gene ID: 12445

Mouse SwissProt Number: P30282

Rat Gene ID: 25193

Rat SwissProt Number: P48961

Research Use Only