Product Description

Cytochrome P450 2D6 (CYP2D6) Activity Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F023-48, E-BC-F023-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Cytochrome P450 2D6 (CYP2D6) is a member of the cytochrome P450 enzyme family that is responsible for metabolizing approximately 25% of drugs in the human body and is primarily expressed in the liver. CYP2D6 has significant metabolic effects on many clinically important drugs, including antidepressants, antipsychotics, beta-blockers, and painkillers. It converts these drugs into more excretable metabolites through oxidation reactions. The key role of CYP2D6 in drug metabolism and the significant influence of individual differences make detecting the activity of this enzyme important for personalized medicine.
The detection principle of this kit: the substrate is converted into a fluorescent substance after catalysis by CYP2D6, and the fluorescence value is detected at the excitation wavelength of 390 nm and the emission wavelength of 468 nm, and the enzyme activity is calculated by the fluorescence value of the standard product.

Abbreviation: CYP2D6

Research Areas: Metabolic Diseases;Liver and Renal Function

Result Type: Enzyme activity

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Detection Range: 0.29-4.42 U/L

Tested Sample Types: Liver microsome

Assay Length: 2 h 20 min

Sensitivity: 0.29 U/L

Inter cv: 9.0-9.9%

Intra cv: 2.1-3.1%

Research Use Only