Product Description

Cytokeratin 18 (phospho Ser52) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6035-50, ES6035-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: KRT18, CYK18, PIG46, Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser52. AA range:21-70

Isotype: IgG

Target: Cytokeratin 18

Specificity: Phospho-Cytokeratin 18 (S52) Polyclonal Antibody detects endogenous levels of Cytokeratin 18 protein only when phosphorylated at S52.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in colon, placenta, liver and very weakly in exocervix, Increased expression observed in lymph nodes of breast carcinoma,

Observed Band (KD): 48kD

Fields: Estrogen signaling pathway, Staphylococcus aureus infection

Function: Disease: Defects in KRT18 are a cause of cryptogenic cirrhosis [MIM: 215600]
Function: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection
Induction: By IL-6
Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic, 40-55 kDa) and II (neutral to basic, 56-70 kDa)
PTM: O-glycosylated at multiple sites, glycans consist of single N-acetylglucosamine residues
PTM: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6
PTM: Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either

Subcellular Location: Cytoplasm, perinuclear region Nucleus, nucleolus

Storage and Stability: -20°C/1 year

Gene Name: KRT18

Protein Name: Keratin type I cytoskeletal 18

Mouse SwissProt Number: P05784

Research Use Only