Product Description

DDR1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7555-50, ES7555-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: DDR1, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, Epithelial discoidin domain-containing receptor 1, Epithelial discoidin domain receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2,

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human DDR1. AA range:749-798

Isotype: IgG

Target: DDR1

Specificity: DDR1 Polyclonal Antibody detects endogenous levels of DDR1 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level), Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney, Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas, Abundant in breast carcinoma cell lines, In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria, In the thyroid gland, expressed in the epithelium of the thyroid follicles, In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas, In kidney, expressed in the epithelia of the distal tubules, Not

Observed Band (KD): 100kD

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Domain: The Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates
Function: May be involved in cell-cell interactions and recognition
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily
Similarity: Contains 1 F5/8 type C domain
Similarity: Contains 1 protein kinase domain
Tissue Specificity: Expressed at low levels in most adult tissues and is highest in the brain and lung. Abundant in breast carcinoma cell lines

Subcellular Location: [Isoform 1]: Cell membrane, Single-pass type I membrane protein, [Isoform 2]: Cell membrane, Single-pass type I membrane protein, [Isoform 3]: Secreted, [Isoform 4]: Cell membrane, Single-pass type I membrane protein

Storage and Stability: -20°C/1 year

Gene Name: DDR1

Protein Name: Epithelial discoidin domain-containing receptor 1

Mouse Gene ID: 12305

Mouse SwissProt Number: Q03146

Rat SwissProt Number: Q63474

Research Use Only