DDX3 Rabbit PolymAb®
Sizes: 20μL, 100μL
Catalogue Numbers: A25442PM-20, A25442PM-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.
Category: Monoclonal Antibodies
Applications: WB, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 73kDa
Observed Molecular Weight: 73kDa
Immunogen: Synthetic peptide .
Species: Human
GeneID: 1654
SWISS: O00571
Alternate Names: DBX; DDX3; HLP2; DDX14; CAP-Rf; MRX102; MRXSSB
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:30000 - 1:180000 IF/ICC, 1:100 - 1:400 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, RNA Binding, Cell Biology Developmental Biology, Stem Cells, Embryonic Stem Cells.
NCBI Alias: DDX3X
Research Use Only