Product Description

DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8091-48, ELK8091-96, ELK8091-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: DH-VD3, Calcitriol, Rocaltrol, Calcijex, 1, 25-(OH)2D3, 1, 25-Dihydroxycholecalciferol, 1, 25-Dihydroxy Vitamin D3

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Metabolic Pathway, Bone Metabolism

Assay Length: 2h

Sensitivity: 48.8 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Research Use Only