DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8091-48, ELK8091-96, ELK8091-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25- (OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3
Assay Type: Competitive Inhibition
Sensitivity: 48.8 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only