DNA pol ι rabbit pAb - ES4614

DNA pol ι rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES4614-50, ES4614-100

Citations, Manuals and MSDS Available upon request.

Background: catalytic activity:Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1)., cofactor:Magnesium., domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity., function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity., similarity:Belongs to the DNA polymerase type-Y family., similarity:Contains 1 umuC domain., subcellular location:Accumulates at replication forks after DNA damage., subunit:Binds REV1L (By similarity). Binds POLH., tissue specificity:Ubiquitous. Highly expressed in testis.,

Alternate Name: POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B

Source: Rabbit

Applications: WB; ELISA

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Reactivity: Human; Rat; Mouse;

Immunogen: The antiserum was produced against synthesized peptide derived from human POLI. AA range:641-690

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 85kD

Human Gene ID: 11201

Human SWISS Prot NO: Q9UNA4

Subcellular Location: Nucleus. Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage.

Research Use Only