Dog ADM (Adrenomedullin) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK0167-48, ELK0167-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide
Species: Dog
Assay Type: Sandwich
Sensitivity: 4.42 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, Plasma and other Biological Fluids.
Assay length: 3.5h
Research Areas: Endocrinology; Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ADM in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.