Product Description

Dog FABP3 (Fatty Acid Binding Protein 3, Muscle And Heart) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11906-48, ELK11906-96, ELK11906-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Dog

Alternate Names: MDGI, FABP11, H-FABP, O-FABP, Hfabp, Mammary Derived Growth Inhibitor, Fatty Acid Binding Protein 11, Muscle fatty acid-binding protein, Heart-type fatty acid-binding protein

Sensitivity: 0.29 ng/mL

Assay Type: Sandwich

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, Plasma, Other Biological Fluids.

Assay Length: 3.5h

Research Area: Metabolic Pathway, Cardiovascular Biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog FABP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog FABP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog FABP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog FABP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only