Dog HbA1c (Glycated Hemoglobin A1c) ELISA Kit - ELK9768

Dog HbA1c (Glycated Hemoglobin A1c) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9768-48, ELK9768-96, ELK9768-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Assay Type: Competitive Inhibition

Sensitivity: 4.07 µg/mL

Standard: 1000 µg/mL

Detection Range: 15.63-1000 µg/mL

Sample Type: plasma and erythrocyte lysates

Assay Length: 2h

Research Areas: Diagnosis of diabetes mellitus

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only