Dog MDA (Malondialdehyde) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9999-48, ELK9999-96, ELK9999-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: MDA
Assay Type: Competitive Inhibition
Sensitivity: 9.23 ng/mL
Standard: 2000 ng/mL
Detection Range: 31.25-2000 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Hepatology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog MDA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog MDA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog MDA in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only