Dog vWFCP (Von Willebrand Factor Cleaving Protease) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9063-48, ELK9063-96, ELK9063-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: ADAMTS13; TTP; vWF-cp; vWF-cleaving protease; A Disintegrin And Metalloproteinase With A Thrombospondin Type 1 Motif Member 13
Assay Type: Sandwich
Sensitivity: 0.126 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Enzyme and Kinase; Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog vWFCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog vWFCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog vWFCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog vWFCP in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only