E2F1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A16720-20, A16720-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 47kDa
Observed Molecular Weight: 70kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 1869
SWISS: Q01094
Alternate Names: RBP3; E2F-1; RBAP1; RBBP3; E2F1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, Transcription Factors, Cancer, Tumor suppressors, Signal Transduction, ErbB-HER Signaling Pathway, Cell Biology Developmental Biology, Apoptosis, Cell Cycle, Cell Cycle Control-G1 S Checkpoint.
NCBI Alias: E2F1
Research Use Only