Product Description

EasyStep Human 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK051ES-48, ELK051ES-96, ELK051ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 25 Hydroxy Vitamin D,

Reactivity: Human

Sample Type: Serum, Plasma

Assay Type: Competitive Inhibition

Research Areas: Reproductive Science, Genetic Science, Nutrition Metabolism, Bone Metabolism

Assay Length: 1.5h

Sensitivity: 7.7 ng/mL

Standard: 5000 ng/mL

Detection Range: 78.13-5000 ng/mL

Research Use Only