EasyStep Human ADP (Adiponectin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK028ES-48, ELK028ES-96, ELK028ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
Assay Type: Sandwich
Sensitivity: 1.9 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Metabolic pathway; Infection immunity; Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADP, and the Human ADP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human ADP. After TMB substrate solution is added, only those wells that contain Human ADP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q15848
Research Use Only