Product Description

EasyStep Human APOB (Apolipoprotein B) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK093ES-48, ELK093ES-96, ELK093ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: Apo-B, FLDB, Apo B-100, Apo B-48

Sensitivity: 43.2 µg/mL

Assay Type: Sandwich

Standard: 12 µg/mL

Detection Range: 0.19-12 µg/mL

Sample Type: Serum, Plasma

Assay Length: 1.5h

Research Area: Metabolic Pathway, Endocrinology, Cardiovascular Biology, Hepatology, Nutrition Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOB, and the Human APOB standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human APOB. After TMB substrate solution is added, only those wells that contain Human APOB and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOB in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only