EasyStep Human hCG (Hyperglycosylated Chorionic Gonadotropin) ELISA Kit - ELK089ES

EasyStep Human hCG (Hyperglycosylated Chorionic Gonadotropin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK089ES-48, ELK089ES-96, ELK089ES-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: hCG; Chorionic Gonadotrophin

Assay Type: Sandwich

Sensitivity: 0.08 mIU/mL

Standard: 500mIU/mL

Detection Range: 7.81-500 mIU/mL

Sample Type: Serum, plasma

Assay Length: 1.5h

Research Areas: Endocrinology; Reproductive science; Neuroscience; Genetic science; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hCG, and the Human hCG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human hCG. After TMB substrate solution is added, only those wells that contain Human hCG and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hCG in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only