Product Description

EasyStep Human IFNg (Interferon Gamma) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK067ES-48, ELK067ES-96, ELK067ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ, and the Human IFN-γ standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IFN-γ. After TMB substrate solution is added, only those wells that contain Human IFN-γ and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFN-γ in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternate Names: IFN gamma, IFN-G, IFG, IFI, INFr, IFN, Immune Interferon

Reactivity: Human

Sample Type: Serum, Plasma, Cell

Assay Type: Sandwich

Research Areas: Cytokine, Infection Immunity

Assay Length: 1.5h

Sensitivity: 15 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Research Use Only