Product Description

EasyStep Human IGFBP1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK079ES-48, ELK079ES-96, ELK079ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP1, and the Human IGFBP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP1. After TMB substrate solution is added, only those wells that contain Human IGFBP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: IBP1, AFBP, IGF-BP25, PP12, Placental Protein 12, Amniotic Fluid Binding Protein, Alpha-Pregnancy-Associated Endometrial Globulin, Growth Hormone Independent-Binding Protein

Reactivity: Human

Sample Type: Serum, Plasma

Assay Type: Sandwich

Research Areas: Tumor Immunity, Endocrinology, Cardiovascular Biology

Assay Length: 1.5h

Sensitivity: 0.54 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Research Use Only