EasyStep Human IGFBP7 (Insulin Like Growth Factor Binding Protein 7) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK062ES-48, ELK062ES-96, ELK062ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: FSTL2; IGFBP-7v; MAC25; PSF; TAF; IGFBP-rP1; MAC25 protein; Prostacyclin-stimulating factor; PGI2-stimulating factor; Tumor-derived adhesion factor
Assay Type: Sandwich
Sensitivity: 1.4 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP7, and the Human IGFBP7 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP7. After TMB substrate solution is added, only those wells that contain Human IGFBP7 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only