EasyStep Human IL-2sRa/CD25 (Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK087ES-48, ELK087ES-96, ELK087ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: IL-2RA; CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Assay Type: Sandwich
Sensitivity: 37.66 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Signal transduction; CD and Adhesion molecule; Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only