Product Description

EasyStep Human IL-2sRa/CD25 (Soluble Interleukin-2 Receptor alpha chain) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK087ES-48, ELK087ES-96, ELK087ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: IL-2RA, CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen, IL2Ra, Interleukin 2 Receptor Alpha

Reactivity: Human

Sample Type: Serum, Plasma

Assay Type: Sandwich

Research Areas: Signal Transduction, Cd and Adhesion Molecule, Tumor Immunity, Infection Immunity

Assay Length: 1.5h

Sensitivity: 37.66 pg/mL

Standard: 6000 pg/mL

Detection Range: 93.75-6000 pg/mL

Research Use Only