Product Description

EasyStep Human IL5 (Interleukin 5) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK080ES-48, ELK080ES-96, ELK080ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL5, and the Human IL5 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL5. After TMB substrate solution is added, only those wells that contain Human IL5 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: IL-5, E-CSF, ESGF, EDF, TRF, Colony-Stimulating Factor, Eosinophil, T-Cell replacing Factor, B Cell Differentiation Factor I, Eosinophil Differentiation Factor

Reactivity: Human

Sample Type: Serum, Plasma, Cell Culture supernatants

Assay Type: Sandwich

Research Areas: Cytokine, Infection Immunity

Assay Length: 1.5h

Sensitivity: 0.15 pg/mL

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Research Use Only