Product Description

EasyStep Human IL8 (Interleukin 8) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK085ES-48, ELK085ES-96, ELK085ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL8, and the Human IL8 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL8. After TMB substrate solution is added, only those wells that contain Human IL8 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: IL-8, CXCL8, AMCF-I, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, SCYB8, TSG1, B-ENAP, Neutrophil-Activating Protein 1, Granulocyte Chemotactic Protein 1

Reactivity: Human

Sample Type: Cell culture supernatants

Assay Type: Sandwich

Research Areas: Cytokine, Infection Immunity

Assay Length: 1.5h

Sensitivity: 3.44 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Research Use Only