EasyStep Human LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK040ES-48, ELK040ES-96, ELK040ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2
Assay Type: Sandwich
Sensitivity: 0.78 ng/mL
Standard: 50 ng/mL
Detection Range: 0.78-50 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Enzyme and Kinase; Metabolic pathway; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LpPLA2, and the Human LpPLA2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human LpPLA2. After TMB substrate solution is added, only those wells that contain Human LpPLA2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LpPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only