Product Description

EasyStep Human LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK040ES-48, ELK040ES-96, ELK040ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LpPLA2, and the Human LpPLA2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human LpPLA2. After TMB substrate solution is added, only those wells that contain Human LpPLA2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LpPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: PLA2G7, PAF-AH, PAFAH, Lp-PLA2, LDL-PLA2, Platelet Activating Factor Acetylhydrolase, Plasma, Phospholipase A2, Group VII, LDL-associated phospholipase A2

Reactivity: Human

Sample Type: Serum, Plasma

Assay Type: Sandwich

Research Areas: Enzyme and Kinase, Metabolic Pathway, Infection Immunity

Assay Length: 1.5h

Sensitivity: 0.78 ng/mL

Standard: 50 ng/mL

Detection Range: 0.78-50 ng/mL

Research Use Only