EasyStep Human LTF (Lactoferrin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK065ES-48, ELK065ES-96, ELK065ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1
Assay Type: Sandwich
Sensitivity: 2.85 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LTF, and the Human LTF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human LTF. After TMB substrate solution is added, only those wells that contain Human LTF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LTF in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only