Product Description

EasyStep Human MCP1 (Monocyte Chemotactic Protein 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK071ES-48, ELK071ES-96, ELK071ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCP1, and the Human MCP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MCP1. After TMB substrate solution is added, only those wells that contain Human MCP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternate Names: CCL2, GDCF2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, Chemokine C-C-Motif Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE

Reactivity: Human

Sample Type: Serum, Plasma, Cell Culture Supernatants

Assay Type: Sandwich

Research Areas: Cytokine, Tumor Immunity, Infection Immunity

Assay Length: 1.5h

Sensitivity: 0.57 pg/mL

Standard: 500 pg/mL

Detection Range: 7.81-500 pg/mL

Research Use Only