EasyStep Human MMP8 (Matrix Metalloproteinase 8) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK070ES-48, ELK070ES-96, ELK070ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase
Assay Type: Sandwich
Sensitivity: 24 pg/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Enzyme and Kinase; Infection immunity; Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP-8, and the Human MMP-8 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MMP-8. After TMB substrate solution is added, only those wells that contain Human MMP-8 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP-8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only