Product Description

EasyStep Human NLRP3 (NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK039ES-48, ELK039ES-96, ELK039ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NLRP3, and the Human NLRP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NLRP3. After TMB substrate solution is added, only those wells that contain Human NLRP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: AVP, AGTAVPRL, AII, AII/AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, MWS, NALP3, PYPAF1, Cryopyrin, Cold Induced Autoinflammatory Syndrome 1, Caterpiller-Like Receptor 1.1

Reactivity: Human

Sample Type: Serum, Plasma

Assay Type: Sandwich

Research Areas: Signal Transduction

Assay Length: 1.5h

Sensitivity: 37 pg/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: D4A523

Research Use Only