Product Description

EasyStep Human NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK035ES-48, ELK035ES-96, ELK035ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: NT-Pro-BNP, N-BNP, ANFB_HUMAN, BNP(1-32), BNP(5-29), BNP-32, Gamma-brain natriuretic peptide, nppb

Reactivity: Human

Sample Type: Serum, Plasma

Assay Type: Sandwich

Research Areas: Endocrinology, Cardiovascular Biology

Assay Length: 1.5h

Sensitivity: 0.94 ng/mL

Standard: 60 ng/mL

Detection Range: 0.94-60 ng/mL

Research Use Only